Biofilm Publications

Recent Published Papers

• Michael W. Harding, Lyriam L. R. Marques, Nick Allan, Merle E. Olson, Brenton Buziak, Patricia Nadworny, Amin Omar, Ronald J. Howard, Jie Feng (2022). Bactericidal Efficacy of Oxidized Silver against Biofilms Formed by Curtobacterium flaccumfaciens pv. flaccumfaciens: https://www.ppjonline.org/journal/view.php?number=2328
• Michael Harding, Patricia Nadworny, Brenton Buziak, Amin Omar, Greg Daniels, Jie Feng (2019). Improved Methods for Treatment of Phytopathogenic Biofilms: Metallic Compounds as Anti-Bacterial Coatings and Fungicide Tank-Mix Partners. Molecules, Volume 24, Issue 12, 2312. DOI: 10.3390/molecules24122312.
• Amin Omar, J. Barry Wright, Gregory Schultz, Robert Burrell, Patricia Nadworny (2017). Microbial Biofilms and Chronic Wounds. Microorganisms 2017, 5(1), 9; doi:10.3390/microorganisms5010009.
• Amin Omar and Patricia Nadworny (2016). Review: Antimicrobial efficacy validation using in vitro and in vivo testing methods. Advanced Drug Delivery Reviews. http://dx.doi.org/10.1016/j.addr.2016.09.003
• Patricia Nadworny, Vanessa Incani, Amin Omar, Graeme Prosperi-Porta, (2015). Ag₅IO₆: Novel anti-biofilm activity of a silver compound with application to medical devices. International Journal of Antimicrobial Agents. Volume 45, Issue 6, Pages 586–593. DOI: http://dx.doi.org/10.1016/j.ijantimicag.2014.09.008
• Mikel Gray, Amin Omar, Brenton Buziak (2014). Can a Stool Management System Prevent Environmental Spread of Clostridium Difficile: A Comparative Trial. American Journal of Infection Control. J Wound Ostomy Continence Nurs. 2014 Sep-Oct;41(5):460-5. doi: 10.1097/WON.0000000000000055
• A.E. Parker, D. K. Walker, D.M. Goeres, N. Allan, M.E. Olson, A. Omar (2014). Ruggedness and reproducibility of the MBEC biofilm disinfectant efficacy test. Journal of Microbiological Methods. Volume 102, July 2014, Pages 55–64. https://doi.org/10.1016/j.mimet.2014.04.013.
• ASTM E2799 – 17 Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay.

Nature Protocols Paper (on the MBEC Assay® Method)

• Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening.

History

• Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device.
• Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents.
• The Calgary Biofilm Device: New technology for rapid determination of antibiotic susceptibilities of bacterial biofilms.
• The MBEC Assay™ system: Multiple equivalent biofilms for antibiotic and biocide susceptibility testing.

Antibiotic, Antimicrobial and Biocide Resistance

• Biofilm bacteria: formation and comparative susceptibility to antibiotics.
• Persister cells mediate tolerance to metal oxyanions in Escherichia coli.
• High-throughput metal susceptibility testing of microbial biofilms.
• Persister cells, the biofilm matrix and tolerance to metal cations in biofilm and planktonic Pseudomonas aeruginosa.
• Inhibition of Xylella fastidiosa biofilm formation via metal chelators.
• Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa.
• Pseudomonas pseudoalcaligenes KF707 upon biofilm formation on a polystyrene surface acquire a strong antibiotic resistance with minor changes in their tolerance to metal cations and metalloid oxyanions.
• Oritavancin kills stationary-phase and biofilm Staphylococcus aureus cells in vitro.
• Antibiotic resistance in gram-negative peritonitis.
• Antibiofilm activities of 1-alkyl-3-methylimidazolium chloride ionic liquids.
• Assessing the antimicrobial activity of zinc oxide thin films using disk diffusion and biofilm reactor.
• Antibiofilm efficacy of nitric oxide-releasing silica nanoparticles.
• Activities of daptomycin and vancomycin alone and in combination with rifampin and gentamicin against biofilm-forming Methicillin-Resistant Staphylococcus aureus isolates in an experimental model of endocarditis.
• In vitro activities of a novel nanoemulsion against Burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species.
• Comparative susceptibility of planktonic and 3-day-old Salmonella Typhimurium biofilms to disinfectants.
• Use of biofilm model systems to study antimicrobial susceptibility.
• Growing Burkholderia pseudomallei in biofilm stimulating conditions significantly induces antimicrobial resistance.

Agricultural Diseases

• Characterization of biofilm formation of Xylella fastidiosa in vitro.
• Biofilms: A new understanding of these microbial communities is driving a revolution that may transform the science of microbiology.

Food Safety

• Development of a standard test to assess the resistance of Staphylococcus aureus biofilm cells to disinfectants.
• In vitro biofilm formation of commensal and pathogenic Escherichia coli strains: Impact of environmental and genetic factors.

MBEC Assay® Protocols

• Characterization of biofilm growth and biocide susceptibility testing of Mycobacterium phlei using the MBEC Assay® system.
• Minimum inhibitory concentration (MIC) versus minimum biofilm eliminating concentration (MBEC) in evaluation of antibiotic sensitivity of gram-negative bacilli causing peritonitis.
• Differences in biofilm and planktonic cell-mediated reduction of metalloid oxyanions.
• Biofilm susceptibility to metal toxicity.
• Metal resistance in Candida biofilms.
• Comparative assessment of the antimicrobial susceptibility of Staphylococcus aureus isolates from bovine mastitis in biofilm versus planktonic culture.
• A novel technique for evaluating the activity of biocides against biofilm bacteria.
• High throughput metal susceptibility testing of microbial biofilms.
• Persister cells, the biofilm matrix and tolerance to metal cations in biofilm and planktonic Pseudomonas aeruginosa.
• Quorum-sensing mutations affect attachment and stability of Burkholderia cenocepacia biofilms.
• The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14.
• Effects of the twin-arginine translocase on the structure and antimicrobial susceptibility of Escherichia coli biofilms.
• Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents.
• Burn wound Infections.
• Pace, J. L., Rupp, M. E., and R. G. Finch. 2006. Biofilm, Infection, and Antimicrobial Therapy. Coagulase-Negative Staphylococci, Chapter 7: 109-155.
• Pace, J. L., Rupp, M. E., and R. G. Finch. 2006. Biofilm, Infection, and Antimicrobial Therapy. Minimal Biofilm Eradication, Chapter 13.
• Pace, J. L., Rupp, M. E., and R. G. Finch. 2006. Biofilm, Infection, and Antimicrobial Therapy. Biofilms: Background, Significance, and Roles of Catheters and Indwelling Devices. Section 1.
• Study of susceptibility of oral bacteria biofilm to traditional Chinese drug preventing preventing caries.
• The impact of a cation channel blocker (furosemide) on Pseudomonas aeruginosa PAO1 biofilm architecture.
• Clinical trials in cystic fibrosis.
• Extended antimicrobial susceptibility assays for Staphylococcus aureus isolate from bovine mastitis growing in biofilms.
• Anti-infectives partnering & deal making.
• Sterile peritonities with monocytic predominance secondary to acute rejection of a previously failed renal allograft.
• Bacterial biofilms in wounds.
• Biofilm formation on bone grafts and bone graft substitutes: Comparison of different materials by a standard in vitro test and microcalorimetry.
• Inactivation of Vibrio anguillarum by attached and planktonic Roseobacter.

If you would like more information in regards to our publications, or are looking for insights into biofilms, contract research, or any other related topic, please feel free to reach out to us.