Publications

Recent Published Papers

• Michael Harding; Patricia Nadworny; Brenton Buziak; Amin Omar; Greg Daniels; Jie Feng (2019). Improved Methods for Treatment of Phytopathogenic Biofilms: Metallic Compounds as Anti-Bacterial Coatings and Fungicide Tank-Mix Partners. Molecules, Volume 24, Issue 12, 2312. DOI: 10.3390/molecules24122312.

• Amin Omar, J. Barry Wright, Gregory Schultz, Robert Burrell, Patricia Nadworny (2017). Microbial Biofilms and Chronic Wounds. Microorganisms 2017, 5(1), 9; doi:10.3390/microorganisms5010009.

• Amin Omar, and Patricia Nadworny (2016). Review: Antimicrobial efficacy validation using in vitro and in vivo testing methods. Advanced Drug Delivery Reviews. http://dx.doi.org/10.1016/j.addr.2016.09.003

• Patricia Nadworny, Vanessa Incani, Amin Omar, Graeme Prosperi-Porta, (2015). Ag5IO6: Novel anti-biofilm activity of a silver compound with application to medical devices. International Journal of Antimicrobial Agents. Volume 45, Issue 6, Pages 586–593. DOI: http://dx.doi.org/10.1016/j.ijantimicag.2014.09.008

• Mikel Gray, Amin Omar, Brenton Buziak (2014) Can a Stool Management System Prevent Environmental Spread of Clostridium Difficile: A Comparative Trial. American Journal of Infection Control. J Wound Ostomy Continence Nurs. 2014 Sep-Oct;41(5):460-5. doi: 10.1097/WON.0000000000000055

• A.E. Parker, D. K. Walker, D.M. Goeres, N. Allan, M.E. Olson, A. Omar (2014) Ruggedness and reproducibility of the MBEC biofilm disinfectant efficacy test. Journal of Microbiological Methods. Volume 102, July 2014, Pages 55–64. https://doi.org/10.1016/j.mimet.2014.04.013.

• ASTM E2799 – 17 Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay.

Nature Protocols Paper (on the MBEC assay)

• 10 June 2010 – Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening.
  Read the full publication at nature.com

History

• Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device
• Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents
• The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms
• The MBEC Assay™ System: multiple equivalent biofilms for antibiotic and biocide susceptibility testing

Antibiotic, Antimicrobial and Biocide Resistance

• Biofilm bacteria: formation and comparative susceptibility to antibiotics.
• Persister cells mediate tolerance to metal oxyanions in Escherichia coli.
• High-throughput metal susceptibility testing of microbial biofilms.
• Persister cells, the biofilm matrix and tolerance to metal cations in biofilm and planktonic Pseudomonas aeruginosa.
• Inhibition of Xylella fastidiosa Biofilm Formation via Metal Chelators.
• Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa.
• Pseudomonas pseudoalcaligenes KF707 upon biofilm formation on a polystyrene surface acquire a strong antibiotic resistance with minor changes in their tolerance to metal cations and metalloid oxyanions.
• Oritavancin Kills Stationary-Phase and Biofilm Staphylococcus aureus Cells In Vitro.
• Antibiotic Resistance in Gram-Negative peritonitis.
• Antibiofilm activities of 1-alkyl-3-methylimidazolium chloride ionic liquids.
• Assessing the antimicrobial activity of zinc oxide thin films using disk diffusion and biofilm reactor.
• Antibiofilm efficacy of nitric oxide-releasing silica nanoparticles.
• Activities of Daptomycin and Vancomycin Alone and in Combination with Rifampin and Gentamicin against Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Isolates in an Experimental Model of Endocarditis.
• In Vitro Activities of a Novel Nanoemulsion against Burkholderia and Other Multidrug-Resistant Cystic Fibrosis-Associated Bacterial Species.
• Comparative susceptibility of planktonic and 3-day-old Salmonella Typhimurium biofilms to disinfectants.
• Use of Biofilm Model Systems to Study Antimicrobial Susceptibility.
• Growing Burkholderia pseudomallei in Biofilm Stimulating Conditions Significantly Induces Antimicrobial Resistance.

Agricultural Diseases

• Characterization of Biofilm formation of Xylella fastidiosa In Vitro.
• Biofilms: A new understanding of these microbial communities is driving a revolution that may transform the science of microbiology
• The Evaluation of Commercially Available Disinfectant Combinations on Biofilms for Use in Slaughter Houses (ppt)

• Marques, L. R., Olson, M. E., Ceri, H., Harding, M. W., Allan, N.D., and Earle,M. L. 2006. Phytopathology. Novel methods for high-throughput screening and efficacy testing of pesticides, fungicides and disinfectants – Identifying anti-biofilm products. 96

Food Safety

• Development of a standard test to assess the resistance of Staphylococcus aureus biofilm cells to disinfectants.
• In Vitro biofilm formation of commensal and pathogenic Escherichia coli strains: Impact of environmental and genetic factors.

MBEC™

• Characterization of biofilm growth and biocide susceptibility testing of Mycobacterium phlei using the MBEC Assay™ system.
• Minimum inhibitory concentration (MIC) versus mimimum biofilm eliminating concentration (MBEC) in evaluation of antibiotic sensitivity of gram-negative bacilli causing peritonitis
• Differences in biofilm and planktonic cell mediated reduction of metalloid oxyanions.
• Biofilm susceptibility to metal toxicity
• Metal resistance in Candida biofilms
• Comparative Assessment of the Antimicrobial Susceptibility of Staphylococcus aureus Isolates from Bovine Mastitis in Biofilm Versus Planktonic Culture.
• A novel technique for evalutating the activity of biocides against biofilm bacteria
• High throughput metal susceptibility testing of microbial biofilms
• Persister cells, the biofilm matrix and tolerance to metal cations in biofilm and planktonic Pseudomonas aeruginosa.
• Quorum-Sensing Mutations Affect Attachment and Stability of Burkholderia cenocepacia Biofilms
• The GacS sensor kinase controls phenotypic reversion of small colony variants isolated from biofilms of Pseudomonas aeruginosa PA14
• Effects of the twin-arginine translocase on the structure and antimicrobial susceptibility of Escherichia coli biofilms
• Quorum-Sensing Mutations Affect Attachment and Stability of Burkholderia cenocepacia Biofilms.
• Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents.
• Burn Wound Infections.
• Pace, J. L., Rupp, M. E., and R. G. Finch. 2006. Biofilm, Infection, and Antimicrobial Therapy.Coagulase-Negative Staphylococci, Chapter 7: 109-155.
• Pace, J. L., Rupp, M. E., and R. G. Finch. 2006. Biofilm, Infection, and Antimicrobial Therapy.Minimal Biofilm Eradication, Chapter 13.
• Pace, J. L., Rupp, M. E., and R. G. Finch. 2006. Biofilm, Infection, and Antimicrobial Therapy.Biofilms: Background, Significance, and Roles of Catheters and Indwelling Devices. Section 1.
• Study of susceptibility of oral bacteria biofilm to traditional Chinese drug preventing preventing caries.
• The Impact of a cation channel blocker (furosemide) on Pseudomonas aeruginosa PAO1 biofilm architecture.
• Clinical trials in cystic fibrosis.
• Extended antimicrobial susceptibility assays for Staphylococcus aureus isolate from bovine mastitis growing in biofilms.
• Anti-Infectives Partnering & Deal Making.
• Sterile Peritonities with Monocytic Predominance Secondary to Acute Rejection of a Previously Failed Renal Allograft.
• Bacterial biofilms in wounds.
• Biofilm formation on bone grafts and bone graft substitutes: Comparison of different materials by a standard in vitro test and microcalorimetry.
• Inactivation of Vibrio anguillarum by attached and planktonic Roseobacter.